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PCR-Dipstick Chromatography for Differential Detection of Carbapenemase Genes Directly in Stool Specimens
Rathina Kumar Shanmugakani1,2,3, Yukihiro Akeda4,2,3, Norihisa Yamamoto1,2,3
1Department of Infection Control and Prevention, Graduate School of Medicine, Osaka University, Osaka, Japan.
Abstract:
A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.
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