Introducing MX Cassettes into Saccharomyces cerevisiae
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1Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina 27710 john.mccusker@duke.edu.
Cold Spring Harbor Protocols
|April 5, 2017
Abstract:
The Saccharomyces cerevisiae genome can be readily and precisely modified with the use of knock out (KO) marker cassettes to delete genes. The most frequently used family of KO cassettes is the MX cassettes. This protocol describes how to use the different types of MX cassettes by selecting for prototrophy, utilization of cytosine or acetamide as a sole nitrogen source, or resistance to one of six different drugs.