Related Experiment Videos
Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody
Shahir S Rizk1, Somnath Mukherjee2, Akiko Koide3,4
1Department of Chemistry and Biochemistry, Indiana University South Bend, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, South Bend, Indiana, USA. srizk@iusb.edu.
Scientific Reports
|April 5, 2017
Abstract:
We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of the enzyme isocitrate dehydrogenase 1 (IDH1). We show that a conformation-specific Fab can reactivate an IDH1 mutant associated with brain tumors. The results show that this strategy is a first step towards developing "activator drugs" for a large number of genetic disorders where mutations have disrupted protein function.