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Biosafety Level 3 setup for multiphoton microscopy in vivo.

D Barlerin1, G Bessière1, J Domingues2,3

  • 1EuroBioconcept SAS, 94380, Bonneuil sur Marne, France.

Scientific Reports
|April 5, 2017
PubMed
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Multiphoton microscopy can now be used safely in biosafety level 3 settings. This advance allows in vivo imaging of dangerous pathogens like Mycobacterium tuberculosis in mouse lungs.

Area of Science:

  • Microscopy and Imaging Technologies
  • Infectious Disease Research
  • Pathogen Biology

Background:

  • Multiphoton microscopy offers valuable in vivo cellular insights.
  • Virulent pathogens and biosafety level 3 (BSL3) environments pose significant limitations.
  • Existing methods restrict advanced imaging in high-containment infectious studies.

Purpose of the Study:

  • To develop a method adapting multiphoton microscopy for BSL3 environments.
  • To demonstrate the feasibility of in vivo imaging within BSL3 regulations.
  • To visualize Mycobacterium tuberculosis infection dynamics in a mouse lung model.

Main Methods:

  • Adaptation of multiphoton microscopy for BSL3 containment.
  • In vivo imaging protocols for infectious disease models.

Related Experiment Videos

  • Application to Mycobacterium tuberculosis lung infection in mice.

    • Main Results:

    • Successful implementation of multiphoton microscopy within BSL3.
    • Demonstration of in vivo visualization of M. tuberculosis infection.
    • Acquisition of cellular-level data on host-pathogen interactions.

    Conclusions:

    • The developed method enables safe, advanced in vivo imaging in BSL3.
    • This technique expands research capabilities for highly virulent pathogens.
    • Visualizing M. tuberculosis infection in the lung offers new research avenues.