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Generating Recombinant Vesicular Stomatitis Viruses for Use as Vaccine Platforms
John B Ruedas1, John H Connor2
1Department of Microbiology and National Emerging Infectious Disease Laboratory, Boston University School of Medicine, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 5, 2017
Abstract:
The unique properties of vesicular stomatitis virus (VSV) make it a promising vaccine platform. With the advent of plasmid-based approaches to generate recombinant VSV viruses that express glycoproteins of other viruses, researchers now have the means to generate vaccine candidates targeting a variety of human pathogens. This chapter gives a general overview of the workings of VSV as a vaccine platform and provides a detailed protocol for the generation of recombinant VSV from plasmids.