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Display of HIV-1 Envelope Protein on Lambda Phage Scaffold as a Vaccine Platform
Jonelle L Mattiacio1, Matt Brewer2, Stephen Dewhurst2
1Saint John Fisher College, 3690 East Ave., Rochester, NY, 14618, USA. jmattiacio@sjfc.edu.
Methods in Molecular Biology (Clifton, N.J.)
|April 5, 2017
Abstract:
The generation of a strong antibody response to target antigens is a major goal for vaccine development. Here we describe the display of the human immunodeficiency virus (HIV) envelope spike protein (Env) on a virus-like scaffold provided by the lambda phage capsid. Phage vectors, in general, have advantages over mammalian virus vectors due to their genetic tractability, inexpensive production, suitability for scale-up, as well as their physical stability, making them an attractive vaccine platform.