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Updated: Aug 10, 2026

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Published on: March 15, 2021
XTEND: Extending the depth of field in cryo soft X-ray tomography
Joaquín Otón1, Eva Pereiro2, José J Conesa1
1Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
Abstract:
We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.
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