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Related Experiment Videos

S152 (CD27). A modulating disulfide-linked T cell activation antigen.

R D Bigler1, Y Bushkin, N Chiorazzi

  • 1Department of Neoplastic Diseases, Hahnemann University, Philadelphia, PA 19102.

Journal of Immunology (Baltimore, Md. : 1950)
|July 1, 1988
PubMed
Summary

The S152 antibody recognizes the CD27 molecule on T cells. Its expression increases upon activation, suggesting a role in T cell immune responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • A subset of peripheral blood T cells expresses a low-density protein recognized by the S152 murine monoclonal antibody (mAb).
  • This determinant is found on both CD4+ and CD8+ T cell subpopulations but not on T cell lines independent of growth factors.

Purpose of the Study:

  • To characterize the S152 antigen and its expression patterns on resting and activated T cells.
  • To investigate the potential functional role of the S152 antigen during T cell activation.

Main Methods:

  • Flow cytometry was used to analyze S152 antigen expression on resting and activated T cells.
  • Mononuclear cells were activated using Concanavalin A (Con A), phytohemagglutinin (PHA), pokeweed mitogen (PWM), or mixed lymphocyte reaction (MLR).
  • Immunoprecipitation and gel electrophoresis were employed to determine the molecular characteristics of the S152 antigen.

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Main Results:

  • S152 antigen expression significantly increased on T cells after activation with Con A or PHA.
  • Activation-induced S152 expression paralleled increases in anti-Tac and 5E9 antigen expression and cellular proliferation.
  • The S152 antigen demonstrated modulation upon activation, decreasing in expression after 2 hours in culture with S152 mAb.
  • Molecular analysis revealed the S152 antigen to be a disulfide-linked homodimer of two 55-kDa molecules.

Conclusions:

  • The S152 mAb identifies the CD27 molecule, a cell surface antigen expressed on resting and activated T cells.
  • The modulation and increased expression of CD27 upon T cell activation suggest a potential functional role in T cell activation processes.