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Chemical and Biological Dynamics Using Droplet-Based Microfluidics.

Oliver J Dressler1, Xavier Casadevall I Solvas1, Andrew J deMello1

  • 1Department of Chemistry and Applied Biosciences, ETH Zürich, CH-8093 Zürich, Switzerland;

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|April 5, 2017
PubMed
Summary

Droplet microfluidics enables dynamic data collection for chemical and biological assays. This review covers droplet formation, manipulation, and analytical methods for exploring previously inaccessible dynamic systems.

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Droplet-based microfluidics are increasingly used in chemical and biological assays.
  • Acquiring dynamic data from microfluidic droplets is challenging due to the need for repeated measurements over extended periods.

Purpose of the Study:

  • To review elemental techniques for microfluidic droplet formation and manipulation.
  • To discuss analytical methods for analyte detection within droplets.
  • To highlight studies utilizing droplet microfluidics for dynamic system characterization.

Main Methods:

  • Review of microfluidic droplet formation and manipulation techniques.
  • Discussion of analytical methods for droplet-based detection.
  • Compilation of case studies demonstrating dynamic system analysis.
Keywords:
dropletshigh-throughput experimentationkineticsmicrofluidicsoptical detection

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Main Results:

  • Recent advancements in droplet formation and manipulation are presented.
  • Various analytical methods suitable for droplet assays are detailed.
  • Successful applications of droplet microfluidics in studying dynamic systems are showcased.

Conclusions:

  • Droplet microfluidics offers powerful tools for dynamic data acquisition.
  • The review provides a comprehensive overview of techniques and applications.
  • This technology opens new avenues for exploring complex dynamic biological and chemical systems.