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A role for planar cell polarity during early endoderm morphogenesis
Lee B Miles1, Takamasa Mizoguchi2, Yutaka Kikuchi3
1School of Biological Sciences, Monash University, Clayton, Victoria 3800, Australia.
Biology Open
|April 6, 2017
Summary
Zebrafish endoderm forms a rod during somitogenesis. Endodermal cells aggregate at the midline without planar cell polarity (PCP) signaling, unlike surrounding tissues.
Area of Science:
- Developmental Biology
- Cell Biology
- Zebrafish Embryogenesis
Background:
- Endoderm development in zebrafish is understood during gastrulation.
- Morphogenic movements of the endoderm during somitogenesis remain poorly understood.
- The transition from a monolayer to a mesenchymal rod is a key developmental event.
Purpose of the Study:
- To characterize endodermal development during zebrafish somitogenesis.
- To describe the morphogenic movements of endoderm formation into a mesenchymal rod.
- To investigate the role of planar cell polarity (PCP) signaling in endoderm migration.
Main Methods:
- Live imaging of zebrafish embryos during somitogenesis.
- Analysis of endodermal cell behavior and polarization.
- Investigating the involvement of PCP signaling pathways.
Main Results:
- Endodermal cells migrate to the midline and aggregate without prior polarization.
- A novel process termed 'midline aggregation' was identified in endodermal cells.
- Planar cell polarity (PCP) signaling is not involved in endodermal midline aggregation during somitogenesis.
Conclusions:
- Endodermal cell migration and midline aggregation during somitogenesis occur independently of PCP signaling.
- Zebrafish endoderm exhibits unique migratory behaviors distinct from mesoderm and ectoderm.
- This study elucidates novel aspects of endoderm morphogenesis during early development.