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Chemically different non-thermal plasmas target distinct cell death pathways.
Oleg Lunov1, Vitalii Zablotskii2, Olexander Churpita2
1Institute of Physics of the Academy of Sciences of the Czech Republic, Prague, 18221, Czech Republic. lunov@fzu.cz.
Scientific Reports
|April 6, 2017
Summary
Non-thermal plasmas (NTPs) trigger distinct cell death pathways. Helium NTPs induce necroptosis, while air NTPs cause mTOR-related necrosis via autophagy inhibition, offering insights into plasma-cell interactions.
Area of Science:
- Biochemistry
- Cell Biology
- Plasma Medicine
Background:
- Non-thermal plasmas (NTPs) show promise in biomedical applications.
- Understanding cellular responses to NTPs is crucial for therapeutic development.
- Distinct NTP chemistries may elicit varied cellular effects.
Purpose of the Study:
- To biochemically analyze and decouple cell death pathways induced by different NTPs.
- To investigate the specific molecular mechanisms underlying NTP-mediated cell death.
- To differentiate cellular responses to helium NTP, air NTP, and ozone.
Main Methods:
- Biochemical analysis of cell death pathways.
- Treatment of cells with helium NTP, air NTP, and ozone.
- Assessment of necrosome formation, necroptosis, mTOR activation, autophagy, reactive oxygen species (ROS) production, and mitochondrial permeability transition (MPT).
Main Results:
- Helium NTP treatment induced necrosome formation and necroptosis.
- Air NTP treatment activated mTOR and inhibited autophagy, leading to mTOR-related necrosis.
- Ozone treatment alone caused significant ROS production, inducing CypD-related necrosis via MPT.
Conclusions:
- NTPs trigger distinct cell death pathways based on their chemical composition.
- Helium NTPs primarily induce necroptosis, while air NTPs induce necrosis via autophagy inhibition.
- Ozone induces necrosis through ROS-mediated mitochondrial damage, highlighting specific plasma-induced cellular responses.