Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

isomiR2Function: An Integrated Workflow for Identifying MicroRNA Variants in Plants.

Kun Yang1, Gaurav Sablok2, Guang Qiao1

  • 1Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region - Ministry of Education, Institute of Agro-bioengineering, Guizhou UniversityGuiyang, China; College of Life Sciences, Guizhou UniversityGuiyang, China.

Frontiers in Plant Science
|April 6, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome assemblies of the liverwort Blasia pusilla uncover a well-defined pseudoautosomal region on homomorphic UV sex chromosomes.

Genome biology·2026
Same author

CAG-targeting artificial miRNA with reduced off-target risk for efficient lowering of pathogenic polyglutamine proteins.

NAR molecular medicine·2026
Same author

RsMYB8-RsMYB73 module positively regulates parthenocarpic fruitsetting via elevating <i>RsGA3ox9</i> expression in seedless chestnut rose (<i>Rosa sterilis</i>).

Horticulture research·2026
Same author

Ectopic overexpression of CpFT from 'Manaohong' cherry: Promoting floral induction and floral organ development in tobacco under rain shelter cultivation.

Plant physiology and biochemistry : PPB·2026
Same author

The HpGIF1-HpABC44 module facilitates heavy metal detoxification via improved ABA sensitivity and ROS scavenging.

Plant science : an international journal of experimental plant biology·2025
Same author

Characterization of MADS-box genes in Chinese cherry and overexpression of CpMADS2 promoting early flowering in transgenic Arabidopsis.

Journal of plant physiology·2025

This study introduces isomiR2Function, a new tool for discovering and classifying plant microRNAs (miRNAs) and their variations (isomiRs). It accurately identifies isomiRs and predicts their functions, aiding plant research.

Area of Science:

  • Plant molecular biology
  • Non-coding RNA research
  • Bioinformatics tool development

Background:

  • MicroRNAs (miRNAs) are crucial non-coding RNAs (ncRNAs) regulating plant gene expression.
  • IsomiRs, miRNA variants, play significant roles, but accurate classification and prediction remain challenging.
  • Existing tools lack comprehensive features for plant isomiR analysis.

Purpose of the Study:

  • To develop a robust, standalone tool, isomiR2Function, for high-throughput discovery and classification of plant isomiRs.
  • To enable identification of differentially expressed isomiRs and predict their targets.
  • To facilitate functional enrichment analysis of predicted targets.

Main Methods:

  • Developed isomiR2Function, a standalone bioinformatics tool.
Keywords:
PARE-seqfunctional profilingisomiRsmiRNAsplantspost-transcriptional machinery

Related Experiment Videos

  • Integrated templated and non-templated isomiR discovery.
  • Incorporated differential expression analysis and target prediction (Targetfinder/Cleaveland).
  • Utilized TopGO for functional enrichment analysis.

    • Main Results:

    • isomiR2Function demonstrated highly accurate prediction and classification of isomiRs.
    • Benchmarking showed superior performance compared to existing tools.
    • The tool streamlines isomiR profiling from discovery to functional characterization.

    Conclusions:

    • isomiR2Function provides a comprehensive solution for plant isomiR analysis.
    • The tool facilitates efficient identification and functional characterization of isomiRs.
    • It simplifies miRNA sequencing data processing for researchers.