Reference Values for the Revised Anti-Müllerian Hormone Generation II Assay: Infertile Population-based Study

Area of Science:

• Reproductive Endocrinology
• Clinical Biochemistry
• Infertility Research

Background:

• Anti-Müllerian hormone (AMH) is a key clinical marker for assessing ovarian reserve.
• AMH testing is a common initial step in infertility evaluations.
• Establishing reliable reference values is crucial for accurate interpretation.

Purpose of the Study:

• To establish population-based reference values for AMH using the revised second generation (Gen II) assay.
• To develop and validate AMH-age regression models for infertile women.
• To provide updated centile charts for AMH levels based on age.

Main Methods:

• Population-based cohort study involving 15,801 infertile women aged 25-45 years.
• AMH levels were measured using the revised Gen II assay.
• Five AMH-age regression models were established and validated, with a quadratic model identified as optimal.

Main Results:

• A quadratic regression model (log AMH = 0.410 × age -0.008 × age² -3.791) best described the age-related decline in AMH.
• The study generated age-specific reference values and centile charts for AMH.
• This represents the largest population-based dataset for establishing AMH reference values with the revised Gen II assay.

Conclusions:

• The established AMH reference values, derived from a large population, enhance the accuracy of ovarian reserve estimation.
• The revised Gen II assay and the developed models provide a more precise tool for clinical practice.
• These findings support improved diagnostic capabilities in infertility management.