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Association study between genetic variations in Axin2 gene and lung cancer risk in North Indian population: A

Charu Bahl1, Siddharth Sharma1, Navneet Singh2

  • 11 Department of Biotechnology, Thapar University, Patiala, India.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|April 6, 2017
PubMed

Insights

Genetic variations in the Axin2 gene, specifically at sites 148 C>T and 1712+19 G>T, show a protective effect against lung cancer in North Indians. These Axin2 polymorphisms influence lung cancer susceptibility and may act as tumor suppressors.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • The Wnt signaling pathway is crucial in human carcinogenesis.
  • Axis inhibition protein 2 (Axin2) antagonizes the Wnt pathway and functions as a tumor suppressor.
  • Genetic variations in Axin2 may influence lung cancer susceptibility.

Purpose of the Study:

  • To investigate the association between seven polymorphic sites in the Axin2 gene and lung cancer risk in a North Indian population.
  • To identify specific Axin2 variants that confer protection or increase susceptibility to lung cancer.

Main Methods:

  • Genotyping of 608 subjects (303 cases, 305 controls) for seven Axin2 polymorphic sites using PCR-RFLP.
  • Association analysis using logistic regression to determine odds ratios and statistical significance.
  • Multivariate analysis including MDR and CART to evaluate gene-gene interactions.

Main Results:

  • Three Axin2 polymorphic sites (148 C>T, 1365 G>A, 1712+19 G>T) demonstrated a protective effect against lung cancer.
  • The Axin2 148 C>T variant (TT genotype) was significantly associated with squamous cell carcinoma (SQCC).
  • Axin2 1712+19 G>T heterozygous genotype (GT) showed decreased risk across all histological subtypes.
  • MDR and CART analyses identified significant interactions between Axin2 148, 2062, and 1712+19 SNPs in modulating lung cancer risk.

Conclusions:

  • Specific Axin2 gene polymorphisms (148 C>T, 1365 G>A, 1712+19 G>T) are associated with altered lung cancer risk in North Indians.
  • Axin2 variants, particularly 148 and 1712+19, play a significant role in modulating lung cancer susceptibility.
  • These findings highlight the potential of Axin2 as a biomarker for lung cancer risk assessment.
Keywords:
Axin2Lung cancerWnt pathwaysingle-nucleotide polymorphism

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