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Related Experiment Videos

A dot-immunobinding assay for infectious bronchitis virus.

M A Muneer1, J A Newman, V Sivanandan

  • 1Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.

Avian Diseases
|January 1, 1988
PubMed
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This study compared Whatman filter paper and nitrocellulose membranes for detecting avian infectious bronchitis virus (AIBV) antibodies. Both methods proved sensitive, economical, and quick for diagnosing AIBV in poultry.

Area of Science:

  • Veterinary Virology
  • Immunodiagnostics

Background:

  • Arkansas avian infectious bronchitis virus (AIBV) poses a significant threat to poultry health.
  • Accurate and efficient diagnostic methods are crucial for controlling AIBV outbreaks.

Purpose of the Study:

  • To compare the sensitivity of Whatman filter paper grade 1 and nitrocellulose membrane for detecting serum antibody titers to AIBV.
  • To evaluate the suitability of a dot-immunobinding assay for AIBV antibody detection.

Main Methods:

  • Dot-immunobinding assay utilizing AIBV antigen adsorbed onto filter paper discs or nitrocellulose membrane.
  • Detection of bound antibodies using rabbit anti-chicken IgG horseradish-peroxidase (HRP) conjugate and a colorimetric substrate (4-chloro-1-naphthol).

Main Results:

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  • Both Whatman filter paper and nitrocellulose membrane demonstrated high sensitivity in detecting AIBV antibodies.
  • The assay requires minimal amounts of antigen and antisera.
  • The dot-immunobinding assay is economical, easy to perform, and yields results within 6-8 hours.

Conclusions:

  • Whatman filter paper grade 1 is a viable and sensitive alternative to nitrocellulose membranes for AIBV antibody detection.
  • The developed dot-immunobinding assay is a practical and efficient tool for avian infectious bronchitis virus diagnosis in veterinary settings.