Related Experiment Video
Updated: Aug 18, 2026

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue
Published on: February 24, 2023
Adjuvant-induced polyarthritis. Synovial cell activation prior to polyarthritis onset
J P López-Bote1, C Bernabeu, A Marquet
1Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Abstract:
We studied synoviocytes obtained from rats that had been injected with Freund's complete adjuvant 12 days prior to killing. We found that several activation parameters were affected in these synoviocytes, namely, the concanavalin A protein-binding pattern, the production of superoxide, and the appearance of the p77 polypeptide, which we have previously shown to be associated with the activation caused by the induction of polyarthritis. Our findings suggest that prior to the establishment of the inflammatory process, synoviocytes are in a state of activation, and may be a component of the molecular mechanism during the early stages of disease.
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