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Related Experiment Videos

[Experimental studies on maedi-visna].

P Russo1, C Vitu, R Vigne

  • 1Ministère de l'Agriculture, Laboratoire National de Pathologie des Petits Ruminants et des Abeilles, Nice, France.

Comparative Immunology, Microbiology and Infectious Diseases
|January 1, 1988
PubMed
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This study investigated sheep lentivirus pathogenicity, finding varied infectivity among strains and routes. Despite viral isolation, clinical signs were absent, suggesting complex factors influence disease development.

Area of Science:

  • Veterinary Virology
  • Immunology
  • Pathogen Biology

Background:

  • Sheep lentiviruses cause significant economic losses in ovine populations.
  • Understanding viral pathogenicity is crucial for developing effective diagnostic and control strategies.
  • Existing diagnostic methods like ELISA require optimization for improved accuracy.

Purpose of the Study:

  • To investigate the pathogenicity of different sheep lentivirus strains in vivo.
  • To generate monospecific antisera for improved diagnostic assays.
  • To optimize Enzyme-Linked Immunosorbent Assay (ELISA) for sheep lentivirus detection.

Main Methods:

  • Conducted three distinct experiments over four years using various sheep lentivirus strains.
  • Inoculated sheep via intratracheal and intracerebral routes to assess pathogenicity and immunogenicity.

Related Experiment Videos

  • Utilized wild-type and temperature-sensitive (ts) mutant strains to evaluate viral replication and disease progression.
  • Monitored seroconversion and viral shedding through buffy coat cell isolation.

    • Main Results:

    • One French maedi-visna strain clone induced clear seroconversion, while others showed varied responses.
    • The K1514 strain demonstrated higher immunogenicity compared to K796 and PPV.
    • Intratracheal inoculation was more effective than intracerebral inoculation for eliciting immune response.
    • Sheep infected with ts mutants showed early viral presence but lower antibody levels than with the parental strain.
    • No clinical signs or pathological lesions were observed despite frequent viral isolations from buffy coat cells.

    Conclusions:

    • Viral pathogenicity and infectivity vary significantly among sheep lentivirus strains and inoculation routes.
    • The absence of clinical signs suggests that factors beyond viral replication influence disease manifestation.
    • Further research is needed to identify enhancing factors contributing to disease development.
    • Not all viral clones are equally infectious, and effective infection may involve multiple virion types.