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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Published on: March 26, 2011
An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived
April D Solomon1, Madison E Hytinen1, Aryn M McClain1
1Department of Forensic Science, Virginia Commonwealth University, PO Box 843079, 1015 Floyd Avenue (Room 2011), Richmond, VA, 23284.
Journal of Forensic Sciences
|April 7, 2017
Summary
Optimizing DNA analysis from archived latent fingerprints is crucial for criminal investigations. This study found that direct cutting, specific extraction kits, and concentration methods significantly improve the chances of obtaining STR profiles from this challenging sample type.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Background:
- Limited knowledge exists on DNA analysis from archived latent fingerprints, specifically touch DNA trapped between adhesive and paper.
- Archived latent fingerprints present unique challenges for DNA profiling due to their age and matrix.
Purpose of the Study:
- To comparatively analyze various collection and analytical methods for an optimized DNA profiling workflow.
- To determine the viability of archived latent fingerprints as a DNA source for criminal investigations.
Main Methods:
- Utilized untreated and treated archived latent fingerprints.
- Compared biological sampling techniques, swab diluents, DNA extraction systems (QIAamp® DNA Investigator Kit), and concentration methods (Centri-Sep™ columns).
- Assessed post-amplification purification methods.
Main Results:
- Direct cutting for sample collection, followed by QIAamp® DNA Investigator Kit extraction and Centri-Sep™ concentration, enhanced the likelihood of obtaining STR profiles.
- 9 out of 10 samples yielded STR profiles, with 7-100% of expected alleles and two full profiles obtained using the optimized workflow.
Conclusions:
- Archived latent fingerprints can be a viable DNA source for criminal investigations, including cold cases.
- Carefully selected DNA profiling procedures are essential for successful analysis of this sample type.
