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On/off-switchable LSPR nano-immunoassay for troponin-T
Md Ashaduzzaman1,2, Swapneel R Deshpande1,2, N Arul Murugan3
1Institute of Advanced Materials, UCS, Teknikringen 4A, Mjärdevi Science Park, SE-58330 Linköping, Sweden.
Scientific Reports
|April 7, 2017
Summary
This study presents a reusable troponin-T immunoassay using localized surface plasmon resonance (LSPR) and a thermosensitive polymer. The novel immunosensor demonstrates temperature-controlled regeneration, addressing a key challenge in biosensing.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Materials Science
Background:
- Regenerating immunosensors remains a significant challenge in diagnostics.
- Existing methods often lack reusability and efficiency.
- Troponin-T (TnT) detection is crucial for diagnosing cardiac conditions.
Purpose of the Study:
- To develop a reusable troponin-T (TnT) immunoassay.
- To utilize localized surface plasmon resonance (LSPR) with gold nanorods (GNRs).
- To engineer temperature-controlled affinity using thermosensitive polymers.
Main Methods:
- Functionalizing gold nanorods (GNRs) with anti-TnT antibodies via thermosensitive poly(N-isopropylacrylamide) (PNIPAAM).
- Employing LSPR to detect TnT binding by monitoring shifts in the plasmonic maximum.
- Utilizing computational modeling (molecular docking, dynamics, free energy calculations) to understand interactions.
Main Results:
- Achieved highly sensitive LSPR detection of TnT (7.6 × 10-15 to 9.1 × 10-4 g/mL).
- Demonstrated temperature-dependent immuno-interaction linked to PNIPAAM conformational changes.
- Successfully regenerated the nano-immunoassay via modest temperature changes.
Conclusions:
- Developed a reusable LSPR-based nano-immunoassay for TnT detection.
- PNIPAAM's thermosensitive nature enables efficient sensor regeneration.
- This approach offers a promising platform for reusable biosensing applications.