Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lightweight dual-backbone network with attentional fusion for wound image classification.

Scientific reports·2026

Same author

Cytochemical Staining Characteristics, Morphology, and Ultrastructural Features of Peripheral Blood Cells From One Captive Blue Iguana (Cyclura lewisi).

Veterinary clinical pathology·2025

Same author

A comparison of machine learning methods for recovering noisy and missing 4D flow MRI data.

International journal for numerical methods in biomedical engineering·2024

Same author

Integrated image and location analysis for wound classification: a deep learning approach.

Scientific reports·2024

Same author

Multi-modal wound classification using wound image and location by deep neural network.

Scientific reports·2022

Same author

Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis.

Nature communications·2022

Search research articles

Related Experiment Video

Updated: Jul 18, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

Three-dimensional reconstruction of highly complex microscopic samples using scanning electron microscopy and optical

Ahmadreza Baghaie¹, Ahmad Pahlavan Tafti², Heather A Owen³

¹Department of Electrical Engineering, University of Wisconsin-Milwaukee, Milwaukee, WI, United States of America.

|April 7, 2017

Summary

This study introduces a new method to create 3D models from 2D Scanning Electron Microscope (SEM) images. This technique enhances understanding of micro-scale sample topology and geometry.

More Related Videos

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Related Experiment Videos

Last Updated: Jul 18, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Area of Science:

Materials Science
Microscopy
Computer Vision

Background:

Scanning Electron Microscopy (SEM) provides 2D images of micro-scale samples.
Current SEM techniques lack depth information, limiting detailed surface analysis.
Reconstructing 3D structures from 2D micrographs is crucial for understanding complex sample geometries.

Purpose of the Study:

To develop a novel and accurate approach for recovering the third dimension from SEM images.
To enable detailed 3D reconstruction of micro-scale samples.
To facilitate the interpretation of surface topology and geometry.

Main Methods:

Multi-view image acquisition of microscopic samples.
Pre/post-processing including sparse feature-based stereo rectification.
Nonlocal-based optical flow estimation for dense matching and depth estimation.

Main Results:

Successful 3D reconstructions of highly complex microscopic samples were achieved.
The proposed approach accurately recovers hidden third-dimensional information.
High-definition 3D printed models of samples can be generated.

Conclusions:

The novel method significantly enhances the interpretation of micro-scale sample topology and geometry.
The technique offers a tangible means for physical understanding through 3D printing.
The approach demonstrates superiority over state-of-the-art methods for complex sample analysis.