Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inception and Development of the Papanicolaou Stain Method.

Nikolaos Chantziantoniou1, Amber D Donnelly, Maheswari Mukherjee

  • 1Division of Anatomic Pathology, Department of Pathology, Sidra Medical and Research Center, Qatar Foundation, Doha, Qatar.

Acta Cytologica
|April 7, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Kymi Odyssey Honorary Ceremony and the Future G.N. Papanicolaou Museum: A Personal Appeal for Support, Enrichment, and Visitation.

Acta cytologica·2024
Same author

The current state of digital cytology and artificial intelligence (AI): global survey results from the American Society of Cytopathology Digital Cytology Task Force.

Journal of the American Society of Cytopathology·2024
Same author

Bioactive metabolites of OMEGA-6 and OMEGA-3 fatty acids are associated with inflammatory cytokine concentrations in maternal and infant plasma at the time of delivery.

Clinical nutrition ESPEN·2024
Same author

Digital cytology part 1: digital cytology implementation for practice: a concept paper with review and recommendations from the American Society of Cytopathology Digital Cytology Task Force.

Journal of the American Society of Cytopathology·2023
Same author

Digital cytology part 2: artificial intelligence in cytology: a concept paper with review and recommendations from the American Society of Cytopathology Digital Cytology Task Force.

Journal of the American Society of Cytopathology·2023
Same author

WHO should adjust its global strategy for cervical cancer prevention.

BMJ global health·2023

The Pap stain method evolved through distinct phases, enhancing cellular transparency and diagnostic accuracy for malignancy detection. This development improved the visualization of cell types and diagnostic confidence.

Area of Science:

  • Cytopathology
  • Histology
  • Staining Techniques

Background:

  • Cytodiagnoses of malignancies rely on analyzing nuclear and cytoplasmic features of stained cells.
  • The Papanicolaou (Pap) stain, introduced in 1942, is crucial for cytodiagnosis.

Purpose of the Study:

  • To explore the historical development, chemical evolution, and application of the Pap stain method.
  • To understand the incremental improvements leading to its diagnostic efficacy.

Main Methods:

  • A comprehensive review of the English scientific literature was conducted.
  • Historical data from 1914 to 1960 was analyzed.

Main Results:

  • Early work involved hematoxylin and eosin staining, correlating cell morphology with endocrinology and histology.
Keywords:
CornificationFixationPap stainPap testPapanicolaou stainVaginal fluid smear

Related Experiment Videos

  • The Pap stain evolved in two phases: enhancing cellular transparency (1933-1942) and refining cytoplasmic staining (1942-1960) with dyes like Orange G and EA.
  • Improvements in fixation and staining led to better visualization of cell types and increased diagnostic confidence.

    • Conclusions:

    • The Pap stain's development is linked to historical scientific advancements.
    • Incremental improvements in cellular transparency, fixation, and cytoplasmic staining were key to the method's evolution and diagnostic utility.