Association of +62 G>A Polymorphism in the Resistin Gene with Type 2 Diabetes Mellitus among Thais: Case-Control...

Resistin gene (RETN) polymorphisms in humans may have a role in the pathogenesis of Type 2 Diabetes Mellitus (T2DM) and insulin resistance. There is still lack of evidence on association between +62 G>A polymorphism in the RETN and T2DM among Thais.

To determine the effect of polymorphisms at +62 G>A of RETN on Thai T2DM.

This matched case control study was conducted with a total of 360 samples from all regions of Thailand (180 Thai new T2DM cases and 180 non-T2DM Thais for control) were enrolled. The RETN +62G>A polymorphism were detected using the Polymerase Chain Reaction (PCR) method. Conditional logistic regression was performed to test the association between +62 G>A polymorphism and T2DM.

Among 360 samples that were enrolled, only 350 samples completed molecular analysis. It was found that GA+AA genotype frequencies in T2DM cases was higher than control by 16% (95% CI: 6.0%, 27.0%, p-value=0.002). After adjustments for possible confounders, multivariable analyses by conditional logistic regression showed that the RETN+62 G>A polymorphism was statistically associated with Thai T2DM (OR_adjusted =1.84, 95% CI: 1.03, 3.31, p-value=0.04). Other factors such as; low educational attainment (OR_adjusted=3.87, 95%CI: 1.60, 9.36), hypertension (OR_adjusted=3.07, 95%CI: 1.56, 6.04), had both obese father and mother (OR_adjusted=1.94, 95%CI: 1.06, 3.56) and triglyceride≥150 (OR_adjusted=2.18, 95% CI: 1.18, 4.02) were statistically associated with Thai T2DM (p-value<0.05). While regular consumption of glutinous rice was found to be a protective factor (OR_adjusted=0.29, 95%CI: 0.13, 0.64).

These findings suggest that RETN polymorphism at position +62 G>A may increase the susceptibility to T2DM in Thais.