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Related Experiment Videos

High resolution top-down experimental strategies on the Orbitrap platform.

Kai Scheffler¹, Rosa Viner², Eugen Damoc³

¹Thermo Fisher Scientific, Im Steingrund 4-6, 63303 Dreieich, Germany.

Journal of Proteomics

|April 8, 2017

Summary

Top-down mass spectrometry (MS) enables comprehensive protein analysis without digestion. This guide offers data acquisition strategies for intact protein characterization on the Orbitrap platform, enhancing biopharmaceutical and biomarker research.

Keywords:

High resolution Intact protein standard mixture Mass spectrometry Orbitrap Top-down

Related Experiment Videos

Area of Science:

Biochemistry and Proteomics
Analytical Chemistry
Mass Spectrometry

Background:

Top-down mass spectrometry (MS) offers in-depth protein characterization via intact molecule fragmentation.
Advancements in MS fragmentation techniques preserve labile post-translational modifications.
Increasing applications in biopharmaceutical quality control, clinical biochemistry, and biomarker discovery.

Purpose of the Study:

To provide a technical overview and guidance for top-down MS data acquisition strategies on the Orbitrap platform.
To detail analysis strategies for single proteins and low-complexity protein mixtures.
To introduce a protein standard mixture for quality control and method validation.

Main Methods:

Utilized top-down mass spectrometry on the Orbitrap platform.
Developed data acquisition strategies for intact proteins and protein mixtures.
Employed a standard mixture of six recombinant proteins for method evaluation.

Main Results:

Presented detailed technical guidance for Orbitrap-based top-down proteomics.
Demonstrated effective fragmentation techniques preserving post-translational modifications.
Introduced a novel intact protein standard for instrument and method quality control.

Conclusions:

Top-down MS on the Orbitrap platform is a versatile tool for comprehensive protein analysis.
The provided strategies and standard sample facilitate high-quality data acquisition for biological samples.
This approach supports advancements in biopharmaceutical characterization and biomarker identification.