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Detection of DNA transcripts of measles genes in multiple sclerosis brain tissue by transfection
L W Greenham1, D Hicks, S Smith
1Department of Microbiology, University of Bristol, U.K.
Abstract:
Transfection studies on DNA obtained from 16 multiple sclerosis (MS) and 13 non-MS brain tissues suggested that 3/16 (19%) MS and 3/13 (23%) non-MS samples contained contaminating measles virus double-stranded RNA, and that 6/16 (38%) MS and 0/13 non-MS samples contained DNA transcripts of measles genes. It is argued that the DNA transcripts most probably originated from dual retrovirus and measles infections in the subjects.
Insights
Measles virus RNA was found in some multiple sclerosis (MS) and non-MS brain tissues. DNA transcripts of measles genes were detected in MS samples, suggesting dual infections.
Area of Science:
- Neuroscience
- Virology
- Immunology
Background:
- The etiology of multiple sclerosis (MS) remains incompletely understood, with viral infections being a potential contributing factor.
- Previous research has explored links between measles virus and neurological conditions, including MS.
Purpose of the Study:
- To investigate the presence of measles virus genetic material in brain tissues from multiple sclerosis (MS) patients and non-MS controls.
- To explore potential viral contributions to MS pathogenesis through molecular analysis of brain tissue.
Main Methods:
- DNA was extracted from 16 multiple sclerosis (MS) and 13 non-MS brain tissue samples.
- Transfection studies were performed to detect measles virus double-stranded RNA and DNA transcripts of measles genes.
Main Results:
- Measles virus double-stranded RNA was detected in 19% of MS samples and 23% of non-MS samples.
- DNA transcripts of measles genes were identified in 38% of MS samples but none of the non-MS samples.
- The presence of measles gene DNA transcripts in MS tissues suggests a potential role for dual retrovirus and measles infections.
Conclusions:
- The findings suggest a higher prevalence of measles virus DNA transcripts in MS brain tissues compared to controls.
- The data support the hypothesis that dual infections involving retroviruses and measles virus may contribute to the development of multiple sclerosis.
