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Updated Reference Genome Sequence and Annotation of Mycobacterium bovis AF2122/97
Kerri M Malone1, Damien Farrell1, Tod P Stuber2
1School of Veterinary Medicine, University College Dublin, Dublin, Ireland.
Genome Announcements
|April 8, 2017
Summary
This study updates the Mycobacterium bovis AF2122/97 genome sequence using multiomics data. Key findings include new coding sequences, corrected annotations, and the confirmed presence of the RD900 locus.
Area of Science:
- Genomics
- Microbiology
- Bioinformatics
Background:
- Mycobacterium bovis causes bovine tuberculosis, a significant zoonotic disease.
- Accurate reference genomes are crucial for understanding pathogen evolution and developing control strategies.
Purpose of the Study:
- To refine and update the reference genome sequence of Mycobacterium bovis AF2122/97.
- To improve the annotation accuracy and identify genomic variations.
Main Methods:
- An integrative multiomics approach was employed for genome sequencing and analysis.
- Bioinformatic tools were used for annotation, SNP calling, and sequence correction.
Main Results:
- The updated genome includes 42 new coding sequences (CDSs) and 14 modified annotations.
- Twenty-six single-nucleotide polymorphism (SNP) corrections were implemented.
- The RD900 locus, previously thought absent, was confirmed to be present in the genome.
Conclusions:
- The updated Mycobacterium bovis genome sequence provides a more accurate and comprehensive resource.
- These genomic refinements enhance our understanding of M. bovis and aid in tuberculosis research.
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