Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurochemical Transmission: Sites of Drug Action01:26

Neurochemical Transmission: Sites of Drug Action

3.5K
Neurochemical transmission, the conduction of electrical impulses between neurons mediated by neurotransmitters, plays a vital role in various physiological processes. Autonomic drugs exert their effects by modulating neurotransmission within the autonomic nervous system. For instance, drugs such as hemicholinium block the precursor uptake necessary for synthesizing acetylcholine, an essential autonomic neurotransmitter. Following synthesis, neurotransmitters are stored in vesicles. Metyrosine...
3.5K
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

1.8K
Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Voltage dynamics of cortical dendrites in vivo.

Nature neuroscience·2026
Same author

Reward magnitude determines reinforcement learning efficiency.

Science (New York, N.Y.)·2026
Same author

A unified photosensitizer platform for <i>in situ</i> DNA-, RNA-, and protein-directed proximity labeling.

bioRxiv : the preprint server for biology·2026
Same author

A series of spontaneously blinking dyes for super-resolution microscopy.

Nature methods·2026
Same author

Luminal surface proteome of the brain vasculature uncovers blood-brain barrier regulators.

Science (New York, N.Y.)·2026
Same author

Evolutionarily conserved neural dynamics across mice, monkeys, and humans.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 2, 2026

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
15:05

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Published on: February 5, 2015

11.6K

Deconstructing behavioral neuropharmacology with cellular specificity.

Brenda C Shields1, Elizabeth Kahuno1, Charles Kim1

  • 1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

Science (New York, N.Y.)
|April 8, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed Drugs Acutely Restricted by Tethering (DART) to precisely target drugs to specific cells. This technique precisely mapped the role of AMPA receptors in Parkinsonian motor deficits in mice.

More Related Videos

Purification of the Dendritic Filopodia-rich Fraction
11:51

Purification of the Dendritic Filopodia-rich Fraction

Published on: May 2, 2019

4.7K
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
10:08

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Published on: July 20, 2022

1.6K

Related Experiment Videos

Last Updated: May 2, 2026

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
15:05

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Published on: February 5, 2015

11.6K
Purification of the Dendritic Filopodia-rich Fraction
11:51

Purification of the Dendritic Filopodia-rich Fraction

Published on: May 2, 2019

4.7K
Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies
10:08

Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies

Published on: July 20, 2022

1.6K

Area of Science:

  • Neuroscience
  • Pharmacology
  • Molecular Biology

Background:

  • Understanding behavior requires studying molecular, cellular, and circuit determinants.
  • Broad protein expression complicates acute manipulation in specific cell types.
  • A method is needed to combine pharmacological precision with cell-type specificity.

Purpose of the Study:

  • To develop a novel technique, Drugs Acutely Restricted by Tethering (DART), for rapid, cell-type-specific drug localization.
  • To investigate the role of AMPA receptors (AMPARs) in the motor deficits associated with Parkinson's disease in mice.
  • To demonstrate the versatility of DART for targeting diverse molecular targets.

Main Methods:

  • Developed DART by combining pharmacology with genetic tools for cell-specific drug delivery without modifying native targets.
  • Created an AMPAR antagonist DART and validated it in neuronal cultures, brain slices, and behaving mice.
  • Applied DART to identify specific neuronal populations and receptor contributions to parkinsonian motor deficits.

Main Results:

  • DART successfully localized drugs to defined cell surfaces, enabling precise manipulation.
  • In parkinsonian mice, AMPARs in indirect spiny projection neurons (iSPNs) and tonically active interneurons (TANs) were causally linked to motor deficits.
  • Akinesia was driven by iSPNs and TANs (D2 cells), while movement execution depended on the ratio of AMPARs in D2 versus D1 cells.
  • A muscarinic antagonist DART was designed, proving the method's applicability to various targets.

Conclusions:

  • DART is a powerful technique for rapid, cell-specific drug delivery, overcoming limitations of traditional methods.
  • AMPARs in specific neuronal populations (iSPNs and TANs) play critical roles in the pathophysiology of Parkinsonian motor deficits.
  • DART technology is adaptable for studying diverse neurological conditions and therapeutic targets.