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Characterization of the mouse met proto-oncogene.
A M Chan1, H W King, E A Deakin
1Chemical Carcinogenesis Section, Chester Beatty Laboratories, London, UK.
Oncogene
|June 1, 1988
Summary
The mouse met proto-oncogene encodes a 1380 amino acid growth factor receptor. Its transcripts are highly expressed in yolk sac, amnion, and kidney during late gestation, and the gene is on chromosome 6.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- The met proto-oncogene is implicated in cellular growth and development.
- Understanding the expression patterns and localization of met is crucial for deciphering its role.
Purpose of the Study:
- To characterize the mouse met proto-oncogene product.
- To investigate the expression profile of met transcripts in the developing mouse conceptus.
- To determine the chromosomal location of the mouse met gene.
Main Methods:
- DNA sequencing of cDNA clones to determine the protein sequence.
- RNA analysis (Northern blotting or similar) to detect met mRNA levels in various fetal tissues.
- Analysis of mouse-hamster hybrid cell lines for gene mapping.
Main Results:
- The mouse met gene encodes a 1380 amino acid protein with structural features of a receptor tyrosine kinase, including intracellular kinase, transmembrane, and extracellular domains.
- High levels of met mRNA were detected in the yolk sac, amnion, and kidney of late-gestation mouse conceptuses.
- The met gene was localized to mouse chromosome 6.
Conclusions:
- The mouse met protein functions as a growth factor receptor.
- Met plays a significant role in specific tissues of the late-gestation mouse conceptus, particularly the yolk sac, amnion, and kidney.
- The chromosomal assignment of met provides a basis for further genetic studies and understanding of its regulation.