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MiRNA153 induces pituitary tumor MMQ cell line apoptosis through down-regulating Skp protein expression
1Department of Neurology, Yantai Yeda Hospital, Yantai, Shandong, China. pengzhangcvb@163.com.
Objective:
Pituitary tumor seriously threatens patient's life. MicroRNAs regulate cell growth and apoptosis. This study aims to investigate the effects of miRNA153 on pituitary tumor MMQ cells proliferation and apoptosis.
Materials And Methods:
Synthetic miRNA153 and control miRNA were transfected to MMQ cell line. Cell proliferation and apoptosis were tested by MTT assay and flow cytometry, respectively. Skp protein expression was detected by Western blot assay. Skp siRNA or Skp plasmid was transfected to MMQ cells transfected by miRNA153 to evaluate the influence on MMQ cell apoptosis.
Results:
MMQ cell proliferation was inhibited and apoptosis was enhanced after miRNA153 transfection. Skp protein level decreased in MMQ cells transfected by miRNA153. Skp interference enhanced MMQ cell apoptosis induced by miRNA153. Skp overexpression restrained MMQ cell apoptosis triggered by miRNA153.
Conclusions:
MiRNA153 transfection suppressed MMQ cell growth and induced apoptosis. MiRNA153 regulated MMQ cell apoptosis through down-regulating Skp protein.
Insights
MicroRNA153 (miRNA153) inhibits pituitary tumor cell growth and promotes apoptosis by downregulating Skp protein. This finding offers a potential therapeutic target for pituitary tumors.
Area of Science:
- Endocrinology
- Molecular Biology
- Oncology
Background:
- Pituitary tumors pose significant health risks.
- MicroRNAs (miRNAs) are key regulators of cell growth and apoptosis.
- Understanding miRNA roles is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the effect of miRNA153 on pituitary tumor MMQ cell proliferation and apoptosis.
- To elucidate the underlying molecular mechanism involving Skp protein.
Main Methods:
- MMQ cell line was transfected with synthetic miRNA153 or control miRNA.
- Cell proliferation assessed by MTT assay; apoptosis by flow cytometry.
- Skp protein expression analyzed via Western blot; functional studies using siRNA/plasmid.
Main Results:
- miRNA153 transfection significantly inhibited MMQ cell proliferation and enhanced apoptosis.
- Skp protein levels were decreased following miRNA153 transfection.
- Modulating Skp expression (siRNA/plasmid) confirmed its role in miRNA153-induced apoptosis.
Conclusions:
- miRNA153 suppresses pituitary tumor cell growth and induces apoptosis.
- The mechanism involves the downregulation of Skp protein by miRNA153.
- miRNA153 represents a potential therapeutic agent for pituitary tumors.