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Related Experiment Video

Updated: Jun 19, 2026

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
12:38

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Published on: December 18, 2008

Enhancing the toolbox to study IL-17A in cattle and sheep.

Sean R Wattegedera1, Yolanda Corripio-Miyar2,3, Yvonne Pang2

  • 1Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik, Scotland, EH26 0PZ, UK. Sean.Wattegedera@moredun.ac.uk.

Veterinary Research
|April 9, 2017
PubMed
Summary

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Developing new methods to detect Interleukin-17A (IL-17A) cytokine expression in ruminant T cells is crucial for livestock vaccine development against infectious diseases. This study successfully created novel techniques for IL-17A detection in cattle and sheep.

Area of Science:

  • Immunology
  • Veterinary Science
  • Molecular Biology

Background:

  • Effective livestock vaccines require methods to detect cytokine expression in T cells.
  • Existing methods for detecting T cell cytokines like IFN-γ, IL-4, and IL-10 in ruminants are established, but IL-17A detection methods are limited.
  • IL-17A plays a critical role in host defense and immune regulation, making its detection vital for understanding ruminant immune responses.

Purpose of the Study:

  • To develop and validate novel methods for detecting Interleukin-17A (IL-17A) expression in T cell subsets of ruminants (cattle and sheep).
  • To enable the investigation of IL-17A-producing T cells, contributing to the advancement of livestock vaccine strategies.
  • To identify specific T cell subsets responsible for IL-17A expression in ruminants.

Main Methods:

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Last Updated: Jun 19, 2026

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
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Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Published on: December 18, 2008

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
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Visualization of IL-22-expressing Lymphocytes Using Reporter Mice

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  • Cloning of bovine and ovine IL-17A cDNAs and expression of recombinant proteins in Chinese Hamster Ovary (CHO) cells.
  • Screening of commercial antibodies for IL-17A detection using transfected CHO cells (intracellularly and in culture supernatants).
  • Development of an ELISA for bovine IL-17A, ELISpot assays for peripheral blood mononuclear cells (PBMCs), and flow cytometry using monoclonal antibodies (mabs) for intracellular IL-17A detection and T cell subset phenotyping (CD4, CD8, WC-1).

Main Results:

  • Successful expression of biologically-active recombinant bovine and ovine IL-17A proteins.
  • Demonstration that an ELISA developed for bovine IL-17A can detect native ovine IL-17A.
  • Identification of specific monoclonal antibodies capable of detecting intracellular IL-17A in PBMCs from cattle and sheep, revealing IL-17A expression in distinct T cell subsets (CD4+, CD8+, WC-1+ in cattle; CD4+, WC-1+ in sheep) upon activation.

Conclusions:

  • Novel techniques for detecting IL-17A expression in ruminant T cells have been successfully developed and validated.
  • These methods facilitate the investigation of IL-17A-producing T cells and the characterization of specific T cell subset activation in cattle and sheep.
  • The developed techniques provide a foundation for advancing research on ruminant immunology and the development of improved livestock vaccines.