Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer.

I E Akerblom1, E P Slater, M Beato

  • 1Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037.

Science (New York, N.Y.)
|July 15, 1988
PubMed
Summary

Steroid hormone receptors can inhibit gene expression. The glucocorticoid receptor negatively regulates the human glycoprotein hormone alpha-subunit gene by binding to DNA, revealing a new mechanism of gene regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transmitted HIV-1 drug resistance in a large international cohort using next-generation sequencing: results from the Strategic Timing of Antiretroviral Treatment (START) study.

HIV medicine·2020
Same author

Preoperative Imaging Overestimates the Tumor Size in Pancreatic Neuroendocrine Neoplasms Associated with Multiple Endocrine Neoplasia Type 1.

World journal of surgery·2017
Same author

Refinement of screening for familial pancreatic cancer.

Gut·2016
Same author

Limited resection of pancreatic cancer in high-risk patients can result in a second primary.

Gut·2015
Same author

Global HIV-1 transmitted drug resistance in the INSIGHT Strategic Timing of AntiRetroviral Treatment (START) trial.

HIV medicine·2015
Same author

Patents and literature.

Applied biochemistry and biotechnology·2013

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Gene Regulation

Background:

  • Steroid hormone receptors are known to activate gene expression via hormone-dependent enhancers.
  • The mechanisms by which steroids inhibit gene transcription remain largely unknown.

Purpose of the Study:

  • To investigate the mechanisms of steroid-mediated gene inhibition.
  • To determine if glucocorticoid receptors can negatively regulate gene expression.

Main Methods:

  • Gene transfer studies were employed to assess gene regulation.
  • Analysis of a specific 52-nucleotide region of the human glycoprotein hormone alpha-subunit gene.
  • Purified glucocorticoid receptor DNA-binding assays were performed.

Main Results:

Related Experiment Videos

  • The glucocorticoid receptor was found to negatively regulate the human glycoprotein hormone alpha-subunit gene.
  • Inhibition was mediated by a 52-nucleotide region critical for cAMP responsiveness and placental-specific expression.
  • Glucocorticoid receptor binding sites were identified overlapping cAMP-responsive elements within this region.

Conclusions:

  • Steroid hormone receptors, specifically glucocorticoid receptors, can actively inhibit gene transcription.
  • This inhibition occurs through interference with other transcription factors at specific DNA regions.
  • A novel mechanism for steroid-mediated negative gene regulation has been elucidated.