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Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment.
Alberto Valdés1, Virginia García-Cañas2, Almudena Pérez-Sánchez3
1Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Nicolas Cabrera 9, 28049, Madrid, Spain.
Journal of Chromatography. A
|April 9, 2017
Summary
Rosemary extract significantly inhibits colorectal cancer progression in vivo by altering 74 key proteins involved in RNA modification and protein synthesis. This study provides novel insights into rosemary
Area of Science:
- Pharmacology
- Proteomics
- Oncology
Background:
- Rosemary (Rosmarinus officinalis) extracts exhibit antiproliferative effects against cancer cells in vitro and in vivo.
- Previous studies highlight rosemary's interaction with multiple molecular targets but lack comprehensive in vivo proteomic analysis.
- Understanding rosemary's molecular mechanisms in vivo is crucial for its therapeutic potential in cancer treatment.
Purpose of the Study:
- To investigate the effects of a polyphenol-enriched rosemary extract on xenograft tumor growth in vivo.
- To conduct the first comprehensive in vivo shotgun proteomic analysis using nano-LC-MS/MS and dimethyl labeling (DML) to identify protein changes.
- To elucidate the molecular mechanisms underlying rosemary extract's anti-cancer activity.
Main Methods:
- Administration of a polyphenol-enriched rosemary extract to xenograft tumor models.
- Shotgun proteomic analysis utilizing nano-LC-MS/MS coupled with stable isotope dimethyl labeling (DML) for quantitative protein profiling.
- Bioinformatic analysis to identify deregulated proteins and affected biological pathways.
Main Results:
- Daily administration of rosemary extract reduced colorectal cancer progression in vivo.
- A total of 74 proteins were found to be differentially expressed in response to the rosemary extract treatment.
- Bioinformatic analysis revealed significant alterations in RNA Post-Transcriptional Modification, Protein Synthesis, and Amino Acid Metabolism pathways.
- The study suggests the inactivation of the oncogene MYC as a potential mechanism of action.
Conclusions:
- Rosemary extract demonstrates significant efficacy in reducing colorectal cancer progression in vivo.
- The applied proteomic methodology is highly effective for simultaneous analysis of numerous protein biomarkers in vivo.
- The findings generate new hypotheses regarding the molecular mechanisms of rosemary extract, particularly its impact on RNA modification, protein synthesis, and MYC activity.