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Detection of l-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates
Marie Cellier1, Arthur L James2, Sylvain Orenga1
1Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
Bioorganic & Medicinal Chemistry Letters
|April 9, 2017
Abstract:
Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detecting l-alanylaminopeptidase activity in microorganisms. An Alizarin-based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions.