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Comparison in docetaxel-loaded nanoparticles based on three different carboxymethyl chitosans
Enhui Zhang1, Ronge Xing2, Song Liu2
1Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China.
International Journal of Biological Macromolecules
|April 9, 2017
Summary
O-carboxymethyl chitosan nanoparticles offer a superior drug delivery system for docetaxel, demonstrating enhanced anti-tumor effects. These nanoparticles show improved cellular accumulation and cytotoxicity compared to other carboxymethyl chitosan formulations.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Drug Delivery Systems
Background:
- Carboxymethyl chitosans (CMCs) are versatile polymers with potential in biomedical applications.
- Developing effective nano-carriers is crucial for improving the delivery and efficacy of anti-tumor drugs like docetaxel (DCT).
Purpose of the Study:
- To prepare and characterize three types of carboxymethyl chitosan nanoparticles (OC-NPs, NC-NPs, NOC-NPs) as nano-carriers for docetaxel.
- To evaluate the in vitro performance and therapeutic potential of these distinct nanoparticle formulations.
Main Methods:
- Fabrication of O-carboxymethyl chitosan (OC), N-carboxymethyl chitosan (NC), and N,O-carboxymethyl chitosan (NOC) nanoparticles.
- Characterization of nanoparticles including drug loading capacity (DC), entrapment efficiency (EE), particle size, zeta potential, and morphology.
- In vitro drug release studies, cellular accumulation assays (flow cytometry), and cytotoxicity assessments on SGC 7901 cells.
Main Results:
- All three CMC types formed nanoparticles with sustained docetaxel release, with OC-NPs exhibiting the fastest release rate.
- OC-NPs demonstrated significantly higher cellular accumulation in gastric carcinoma cells compared to NC-NPs and NOC-NPs.
- OC-NPs exhibited superior cytotoxicity against SGC 7901 cells, indicating enhanced anti-tumor efficacy.
Conclusions:
- Physicochemical properties of CMC-based nanoparticles significantly influence their therapeutic effects.
- O-carboxymethyl chitosan nanoparticles represent a promising and more desirable nano-carrier system for docetaxel delivery compared to NC and NOC formulations.