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Updated: Jul 12, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Photosynthetic hydrogen production by alginate immobilized bacterial consortium
Huan Zhang1, Guanyi Chen2, Quanguo Zhang3
1School of Environmental Science & Engineering, Tianjin University, Tianjin 300350, PR China; Collaborative Innovation Center of Biomass Energy, Henan Agricultural University, Zhengzhou 450002, PR China.
Abstract:
Photosynthetic hydrogen production from organic wastewaters using immobilized mixed culture with photosynthetic bacteria (PSB) was studied. A PSB consortium was immobilized by alginate matrix to form granules. The so-yielded granules exhibited minimal diffusional resistances to substrates and to illumination penetration but still produced more hydrogen from synthetic wastewater than the free cells at identical experimental conditions. Optimal granule size, cell loadings, and cell ages for granules and the minimum substrate concentration and maximum illumination intensity requited to maximize hydrogen production were studied. The applied alginate matrix can provide shield to embedded cells from external challenges, likely the produced proton gradients from the surroundings.
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