Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Study on the excision and integration mediated by class 1 integron in Enterococcus faecalis.

Guangchao Yu1, Congxiu Ye2, Qiang Fu1

  • 1Clinical LaboratoryCenter, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.

Microbial Pathogenesis
|April 10, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in Functional Genomics of Disease Resistance in Cucumber (<i>Cucumis sativus</i>) and Translational Prospects for the Cucurbitaceae Family.

Genes·2026
Same author

A novel ZIC1-BMP4 axis identified in photoaged dermal fibroblasts regulates melanogenesis.

Journal of dermatological science·2026
Same author

A comprehensive study on the dual species biofilm formation of clinical <i>Staphylococcus aureus</i> and <i>Candida albicans</i> strains from the same origins.

Biofilm·2025
Same author

Epigenomic Transcriptome Regulation of Growth and Development and Stress Response in Cucurbitaceae Plants: The Role of RNA Methylation.

Current issues in molecular biology·2025
Same author

Exogenous Spermidine Induces Cadmium Stress Tolerance in Cucumber Seedlings by Promoting Plant Growth and Defense System.

Toxics·2025
Same author

Exogenous Sugar Alleviates Salt Stress in Cucumber Seedlings by Regulating the Antioxidant System and Hormone Signaling.

Current issues in molecular biology·2025

Integrons, mobile genetic elements, facilitate gene transfer in bacteria. This study confirms class 1 integron excision and integration in Enterococcus faecalis, highlighting their role in antibiotic resistance in Gram-positive bacteria.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Integrons are mobile genetic elements known to mediate the acquisition and dissemination of exogenous genes, particularly antibiotic resistance genes.
  • Most research on integrons has focused on Gram-negative bacteria, with limited understanding of their role in Gram-positive bacteria.
  • Enterococcus species are significant opportunistic pathogens, and understanding their mobile genetic elements is crucial for combating antimicrobial resistance.

Purpose of the Study:

  • To investigate the excision and integration capabilities of the class 1 integron in the Gram-positive bacterium Enterococcus faecalis.
  • To confirm the role of integrons as potential mediators of antibiotic resistance gene spread in Enterococcus.

Main Methods:

  • Utilized eight plasmids to establish transformants of Enterococcus faecalis for experimental analysis.
Keywords:
AttsiteEnterococcusfaecalisExcisionGene cassetteIntegrationIntegron

Related Experiment Videos

  • Performed excision and integration assays to assess the mobility of the class 1 integron.
  • Employed Polymerase Chain Reaction (PCR) amplification for confirmation of genetic events.

    • Main Results:

    • Demonstrated a positive excision assay for the class 1 integron in Enterococcus faecalis.
    • Subsequent integration assays and PCR amplification confirmed the excision and integration activity.
    • Provided evidence for class 1 integron-mediated mobility of antibiotic resistance genes.

    Conclusions:

    • Class 1 integrons are capable of mediating the excision and integration of exogenous genes in Gram-positive bacteria, specifically Enterococcus faecalis.
    • Integrons represent a significant mechanism for the spread of antibiotic resistance determinants in Enterococcus and other Gram-positive pathogens.
    • These findings underscore the importance of considering integrons as key players in the evolution of antibiotic resistance in clinical settings.