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Related Experiment Videos

Development and validation of a novel multiplexed DNA analysis system, InnoTyper® 21.

Hiromi Brown1, Robyn Thompson1, Gina Murphy1

  • 1InnoGenomics Technologies, LLC, 1441 Canal Street, Suite 307, New Orleans, LA 70112, USA.

Forensic Science International. Genetics
|April 10, 2017
PubMed
Summary

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This study introduces a novel multiplexed DNA analysis system using 20 Alu markers for degraded forensic samples. The InnoTyper 21 system offers high sensitivity and discrimination power for human identification.

Area of Science:

  • Forensic Genetics
  • Molecular Biology
  • Human Identification

Background:

  • Highly degraded DNA samples pose significant challenges in forensic analysis.
  • Short interspersed nuclear elements (SINEs), specifically Alu elements, are abundant in the human genome and offer potential for forensic markers.
  • Previous use of Alu elements in forensics was limited by amplicon size and multiplexing difficulties.

Purpose of the Study:

  • To develop and validate a novel multiplexed DNA analysis system for highly degraded forensic samples.
  • To overcome limitations of previous Alu marker applications by designing primers for small amplicon sizes.
  • To assess the performance of the new system, InnoTyper 21, in sensitivity, specificity, and discriminatory power.

Main Methods:

  • Development of a multiplexed system (InnoTyper 21) comprising 20 bi-allelic Alu markers (INNULs) and Amelogenin.
Keywords:
AluDegraded DNAInnoTyper(®) 21Novel forensic markers

Related Experiment Videos

  • Primer design focused on generating small amplicons (60-125bp) suitable for degraded DNA.
  • Validation studies included PCR optimization, species specificity, sensitivity, degradation, inhibition, precision, accuracy, nonprobative samples, mixtures, and population genetics.

    • Main Results:

    • The InnoTyper 21 system successfully analyzed highly degraded DNA samples, including hair shafts.
    • Sensitivity studies showed allele recovery from as low as 50pg and partial profiles from 25pg of input DNA.
    • Population studies (592 samples across five populations) yielded a random match probability greater than 1 in 3.8 million for the 20 Alu markers.

    Conclusions:

    • The novel primer design enables multiplexed analysis of Alu markers, making them practical for forensic applications.
    • The InnoTyper 21 system demonstrates high sensitivity and discriminatory power, outperforming traditional haplotype systems for degraded samples.
    • This system is a valuable tool for human identification in challenging forensic cases involving degraded DNA, such as missing persons investigations.