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Abolition of DNA recognition site resistance to the restriction endonuclease EcoRII
D H Krüger1, G J Barcak, H O Smith
1Institute of Virology, Humboldt University School of Medicine, Berlin, G.D.R.
Biomedica Biochimica Acta
|January 1, 1988
Abstract:
The EcoRII recognition sites 5'-CCATGG-3' which occur three times in T3 DNA are refractory to this restriction endonuclease. However, these sites are specifically cleaved by EcoRII when a second, susceptible DNA species (pBR 322, phage lambda) is present.