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Updated: Aug 14, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors
Published on: July 31, 2014
Both PepT1 and GLUT Intestinal Transporters Are Utilized by a Novel Glycopeptide Pro-Hyp-CONH-GlcN
1Department of Agricultural, Food and Nutritional Science, University of Alberta , 410 Agriculture/Forestry Centre, Edmonton, AB T6G 2P5, Canada.
Abstract:
Pro-Hyp (PO) accounts for many beneficial biological effects of collagen hydrolysates for skin and bone health. The objective of this study was to conjugate PO with glucosamine (GlcN) to create a novel glycopeptide Pro-Hyp-CONH-GlcN (POGlcN) and then to investigate the potential involvement of multiple transepithelial transport pathways for this glycopeptide. Nuclear magnetic resonance results revealed the amide nature of this glycopeptide with α and β configurations derived from GlcN. This glycopeptide was very resistant to simulated gastrointestinal digestion. Also, it showed a rate of transepithelial transport [permeability coefficient (Papp) of (2.82 ± 0.15) × 10-6 cm/s] across the Caco-2 cell monolayer superior to those of parental dipeptide PO and GlcN [Papp values of (1.45 ± 0.17) × 10-6 and (1.87 ± 0.15) × 10-6 cm/s, respectively]. A transport mechanism experiment indicated that the improved transport efficiency of POGlcN is attributed to the introduction of glucose transporters.
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