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Antibiogramj: A tool for analysing images from disk diffusion tests.

C A Alonso1, C Domínguez2, J Heras2

  • 1Biochemistry and Molecular Biology Area, University of La Rioja, Ed. Científico Tecnológico-CCT. C/ Madre de Dios, 53, Logroño, 26006, Spain.

Computer Methods and Programs in Biomedicine
|April 11, 2017
PubMed
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A new open-source software, AntibiogramJ, semi-automates the measurement of antimicrobial susceptibility zones from disk diffusion tests, improving accuracy and efficiency in microbiology labs.

Area of Science:

  • Microbiology
  • Computer Vision
  • Medical Diagnostics

Background:

  • Disk diffusion testing (antibiogram) is crucial for determining antimicrobial susceptibility.
  • Manual measurement of inhibition zones is time-consuming and prone to errors.
  • Existing automated readers are often expensive and disruptive to lab workflows.

Purpose of the Study:

  • To develop a user-friendly, cost-effective software tool for semi-automated antibiogram analysis.
  • To simplify and improve the accuracy of inhibition zone measurements.
  • To provide an accessible solution for microbiology laboratories.

Main Methods:

  • Designed a software tool, AntibiogramJ, using standard computer vision techniques.
  • The software processes images from disk diffusion tests to semi-automate zone measurement.
Keywords:
AntibiogramsDisk diffusion testImageJInhibition zone readerLearning systemOpenCV

Related Experiment Videos

  • Implemented in Java, compatible with images from various camera devices.

    • Main Results:

    • AntibiogramJ semi-automatically determines, measures, and categorizes inhibition zones.
    • Achieves 87% agreement with expert microbiologists in fully automatic mode.
    • Includes manual correction features for improved accuracy.

    Conclusions:

    • AntibiogramJ is a user-friendly, open-source, and free software for antibiogram analysis.
    • It requires no new equipment or workflow changes in the laboratory.
    • Offers a complete solution for antibiogram analysis, enhancing accessibility and efficiency.