Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HLA expression in human hepatocellular carcinoma.

A C Paterson1, R Sciot, M C Kew

  • 1Experimental Pathology Unit, School of Pathology, South African Institute for Medical Research, Johannesburg.

British Journal of Cancer
|April 1, 1988
PubMed
Summary

Human hepatocellular carcinoma (HCC) frequently shows enhanced expression of MHC class I antigens, but variable expression of MHC class II. This altered antigen presentation may impact tumor behavior.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The surgical pathology laboratory in Mwanza, Tanzania: a survey on the reproducibility of diagnoses after the first years of autonomous activity.

Infectious agents and cancer·2017
Same author

Management of chronic hepatitis C.

Haemophilia : the official journal of the World Federation of Hemophilia·2016
Same author

Review article: 2014 UK consensus guidelines - hepatitis C management and direct-acting anti-viral therapy.

Alimentary pharmacology & therapeutics·2014
Same author

Impact of age on viral kinetics of peginterferon alfa-2a/ribavirin in chronic hepatitis C: final analysis from the PROPHESYS cohort.

Journal of viral hepatitis·2013
Same author

Ductopenia and fetal liver-like architecture as unique and evocative sign of Turner syndrome.

European review for medical and pharmacological sciences·2013
Same author

A practical guide for the use of boceprevir and telaprevir for the treatment of hepatitis C.

Journal of viral hepatitis·2012

Area of Science:

  • Immunology
  • Oncology
  • Molecular Biology

Background:

  • MHC class I and II antigens are crucial for immune surveillance.
  • Their expression patterns in hepatocellular carcinoma (HCC) are not fully understood.
  • Invariant chains play a role in MHC class II antigen presentation.

Purpose of the Study:

  • To investigate the expression of MHC class I and II antigens and invariant chains in HCC.
  • To correlate antigen expression with tumor characteristics.
  • To explore potential implications for tumor behavior.

Main Methods:

  • Analysis of 70 human HCC cases.
  • Immunohistochemical staining using monoclonal and polyclonal antibodies.
  • Evaluation of MHC class I (HLA-A, B, C), beta 2-microglobulin, MHC class II (HLA-DR, DQ), and invariant (I) chain expression.

Related Experiment Videos

Main Results:

  • Over 94% of HCCs showed enhanced or acquired MHC class I (HLA-A, B, C) expression, often with beta 2-microglobulin.
  • HLA-DR expression was observed in 44.9% of cases, typically sparse and heterogeneous.
  • Invariant (I) chain was present in 47.1% of cases, often with intensive and extensive staining.
  • MHC antigen display was independent of tumor differentiation or size.

Conclusions:

  • The majority of HCCs exhibit altered MHC class I expression.
  • Variable and often limited MHC class II and invariant chain expression were noted.
  • Acquisition of MHC class I antigens by HCC may influence tumor behavior and immune evasion.