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Related Experiment Videos

Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection.

Qingwen Zhang1, Lin Wu2, Ten It Wong3

  • 1School of Ophthalmology and Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People's Republic of China.

International Journal of Nanomedicine
|April 11, 2017
PubMed
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This study enhances biosensing sensitivity by combining localized surface plasmon (LSP) and propagating surface plasmon (PSP) on gold nanohole arrays. This novel approach significantly improves fluorescence detection for biomolecules like prostate-specific antigen.

Area of Science:

  • Nanophotonics
  • Biosensing
  • Surface Plasmon Resonance

Background:

  • Localized surface plasmon (LSP) enhances fluorescence but has limited penetration depth, hindering sensitivity for biomolecule detection.
  • Existing LSP-based biosensors struggle with detecting large molecules due to insufficient sensitivity over the entire sensor chip.

Purpose of the Study:

  • To develop a highly sensitive biosensing method by simultaneously exciting localized surface plasmon (LSP) and propagating surface plasmon (PSP).
  • To improve fluorescence enhancement and detection limits for biomolecules using a gold nanohole array.

Main Methods:

  • Simultaneous excitation of LSP and PSP modes on a gold nanohole array using Kretschmann configuration.
  • Detection of prostate-specific antigen (PSA) via a sandwich immunoassay.
Keywords:
fluorescence enhancementgold nanohole arraylocalized surface plasmonpropagating surface plasmonprostate-specific antigen

Related Experiment Videos

  • Utilizing the combined advantages of LSP's field enhancement and PSP's large surface area probing.

    • Main Results:

    • Simulations showed a maximum electric field intensity enhancement of up to 1,600 times under simultaneous LSP and PSP excitation.
    • The sandwich immunoassay for PSA achieved a limit of detection of 140 fM with coexcited LSP and PSP modes.
    • This limit of detection was sevenfold lower compared to using only LSP excitation.

    Conclusions:

    • Coexcitation of LSP and PSP modes significantly enhances fluorescence, leading to highly sensitive bioassays.
    • The gold nanohole array platform offers a promising approach for sensitive detection of various biomolecules.
    • This method provides a pathway for developing next-generation, ultrasensitive biosensing platforms.