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Cinnamaldehyde and eugenol change the expression folds of AKT1 and DKC1 genes and decrease the telomere length of
Abdorrahim Absalan1, Seyed Alireza Mesbah-Namin1, Taki Tiraihi2
1Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Objectives:
To investigate the effect of cinnamaldehyde and eugenol on the telomere-dependent senescence of stem cells. In addition, to search the probable targets of mentioned phytochemicals between human telomere interacting proteins (TIPs) using in silico studies.
Materials And Methods:
Human adipose derived stem cells (hASCs) were studied under treatments with 2.5 µM/ml cinnamaldehyde, 0.1 µg/ml eugenol, 0.01% DMSO or any additive. The expression of TERT, AKT1 and DKC1 genes and the telomere length were assessed over 48-hr treatment. In addition, docking study was conducted to show probable ways through which phytochemicals interact with TIPs.
Results:
Treated and untreated hASCs had undetectable TERT expression, but they had different AKT1 and DKC1 expression levels (CI=0.95; P<0.05). The telomere lengths were reduced in phytochemicals treated with hASCs when compared with the untreated cells (P<0.05). Docking results showed that the TIPs might be the proper targets for cinnamaldehyde and eugenol. Data mining showed there are many targets for cinnamaldehyde and eugenol in the intracellular environment.
Conclusion:
The general effect of cinnamaldehyde and eugenol is their induction of stem cell senescence. Therefore, they could be applicable as chemo-preventive or antineoplastic agents.
Insights
Cinnamaldehyde and eugenol induce stem cell senescence by shortening telomeres. These natural compounds may serve as chemo-preventive or antineoplastic agents.
Area of Science:
- Stem cell biology
- Molecular biology
- Pharmacology
Background:
- Telomere maintenance is crucial for stem cell function.
- Cellular senescence, a state of irreversible growth arrest, is linked to aging and disease.
- Phytochemicals are plant-derived compounds with potential therapeutic properties.
Purpose of the Study:
- To investigate the impact of cinnamaldehyde and eugenol on stem cell senescence.
- To identify potential molecular targets of these phytochemicals within human telomere interacting proteins (TIPs) using in silico methods.
Main Methods:
- Human adipose-derived stem cells (hASCs) were treated with cinnamaldehyde and eugenol.
- Gene expression of TERT, AKT1, and DKC1, along with telomere length, were analyzed.
- Molecular docking studies were performed to predict interactions between phytochemicals and TIPs.
Main Results:
- Cinnamaldehyde and eugenol treatments led to reduced telomere length in hASCs.
- Differential expression of AKT1 and DKC1 genes was observed.
- In silico analysis suggested that TIPs are potential targets for cinnamaldehyde and eugenol.
Conclusions:
- Cinnamaldehyde and eugenol promote stem cell senescence.
- These findings suggest potential applications for cinnamaldehyde and eugenol as chemo-preventive or antineoplastic agents.