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Miniaturized Sample Preparation for Transmission Electron Microscopy

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

Micro/extended-nano sampling interface from a living single cell.

L Lin¹, K Mawatari¹, K Morikawa¹

¹Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. kitamori@icl.t.u-tokyo.ac.jp.

|April 11, 2017

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Researchers developed a new femtoliter sampling technique for living single cells. This method enables precise intracellular analysis while maintaining cell viability, overcoming a key challenge in single-cell studies.

Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Single-cell analysis is crucial but limited by ultra-small sample volumes.
Existing methods struggle with maintaining cell viability during sampling.
Analysis of single molecules within cells requires highly precise sampling techniques.

Purpose of the Study:

To develop a novel femtoliter sampling method for living single cells.
To enable precise intracellular analysis while preserving cell viability.
To address the limitations of current single-cell sampling technologies.

Main Methods:

Utilized micro/nanofluidic device technology for sampling.
Developed a femtoliter-scale sampling technique.
Performed sampling from living human aortic endothelial cells.

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Main Results:

Successfully sampled 39 fL of cytoplasm from a single cell.
Demonstrated the viability of the cell post-sampling.
Validated the femtoliter sampling method for live single-cell analysis.

Conclusions:

The proposed femtoliter sampling method is compatible with maintaining single-cell viability.
This technique advances the field of single-cell analysis by enabling precise intracellular sampling.
Micro/nanofluidic technology offers a promising solution for live single-cell analysis.