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Isolation and characterization of restriction endonuclease BstYI from Bacillus stearothermophilus Y406
FEBS Letters
|July 4, 1988
Abstract:
BstYI, an isoschizomer of XhoII and MflI, has been purified from Bacillus stearothermophilus Y406. This enzyme recognized 5'...Pu/GATCPy...3' in DNA and cleaved between Pu and G in this sequence. BstYI can be easily isolated and purified by heparin-agarose column chromatography in a high yield (8000 units BstYI can be obtained per g wet wt of cells).