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Gene cloning from yeast chromosome-specific mini-library. Isolation of the SRP1-related DNA sequence located on
D Marguet1, E Fantino, G J Lauquin
1Laboratoire de Physiologie Cellulaire, Faculté des Sciences de Marseille-Luminy, Université d'Aix-Marseille, France.
Abstract:
We have isolated and purified chromosome XV DNA molecules from the yeast S. cerevisiae using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. A chromosome-specific mini-library was constructed and the element of the SRP1 (serine-rich protein) related sequence family located within chromosome XV was isolated by in situ colony hybridization with an SRP1 probe. Results indicated that (i) a single-copy sequence homologous to SRP1 is present within chromosome XV; (ii) this sequence lies within the 2.3 kb HindIII fragment of the plasmid pXVAc6; (iii) the lack of a specific transcript from this SRP1-related element suggests that it could be considered as a pseudogene.