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Updated: Jun 20, 2026

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
Published on: December 12, 2013
Chiral Recognition by Dissolution DNP NMR Spectroscopy of 13C-Labeled dl-Methionine
Eva Monteagudo1, Albert Virgili2, Teodor Parella1
1Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona , E-08193 Cerdanyola del Vallès, Barcelona, Spain.
Abstract:
A method based on d-DNP NMR spectroscopy to study chiral recognition is described for the first time. The enantiodifferentiation of a racemic metabolite in a millimolar aqueous solution using a chiral solvating agent was performed. Hyperpolarized 13C-labeled dl-methionine enantiomers were differently observed with a single-scan 13C NMR experiment, while the chiral auxiliary at thermal equilibrium remained unobserved. The method developed entails a step forward in the chiral recognition of small molecules by NMR spectroscopy, opening new possibilities in situations where the sensitivity is limited, for example, when a low concentration of analyte is available or when the measurement of an insensitive nucleus, like 13C, is required. The advantages and current limitations of the method, as well as future perspectives, are discussed.
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