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An Efficient Microscale Technique for Determining the Erythrocyte Sedimentation Rate.

Akhil Chaturvedi1, Sujith Kumar Nagaraj2, Sai Siva Gorthi3

  • 11 Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

SLAS Technology
|April 11, 2017
PubMed
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A new microfluidic method tracks red blood cell (RBC) settling velocity, offering a faster, automated alternative to the erythrocyte sedimentation rate (ESR) test for detecting inflammation. This technique uses minimal blood and provides detailed cell behavior insights.

Area of Science:

  • Biomedical Engineering
  • Hematology
  • Microfluidics

Background:

  • The erythrocyte sedimentation rate (ESR) is a standard diagnostic tool for inflammatory conditions.
  • Conventional ESR testing is time-consuming, requires large blood volumes, and lacks cellular detail.
  • Variability in red blood cell size and aggregation impacts ESR accuracy.

Purpose of the Study:

  • To develop a microfluidic microscopy-based assay for quantifying red blood cell settling velocity.
  • To establish a rapid, automated surrogate for the erythrocyte sedimentation rate (ESR).
  • To overcome the limitations of traditional ESR testing.

Main Methods:

  • A microfluidic channel was used to image individual red blood cells (RBCs) settling vertically.
  • A hybrid algorithm combining eigenvalue background subtraction, centroid detection, Kalman filter, and Hungarian algorithm was employed for cell tracking.
Keywords:
Kalman filtercell trackingerythrocyte sedimentation ratemicrofluidicsred blood cells

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  • The system was optimized for tracking RBCs and aggregates in high cellularity samples.

    • Main Results:

    • The developed microfluidic protocol accurately quantifies RBC settling velocity as an ESR surrogate.
    • Detection accuracy of the cell tracking algorithm reached 79.3%, comparable to existing methods.
    • The approach demonstrated advantages in automation, reduced sample volume (microliters), and rapid results.

    Conclusions:

    • Microfluidic tracking of RBC settling velocity offers a promising, advanced alternative to conventional ESR testing.
    • This method provides a more efficient and informative approach for screening inflammatory conditions.
    • The technology enables faster diagnostics with reduced sample requirements and enhanced data on cell dynamics.