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Generation and characterization of two iPSC lines from human epicardium-derived cells
Christina Paulitschek1, Peggy Schulze-Matz2, Julia Hesse1
1Department of Molecular Cardiology, Medical Faculty, Heinrich Heine University, 40225 Duesseldorf, Germany.
Stem Cell Research
|April 12, 2017
Abstract:
Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.