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Generation of human iPSCs from urine derived cells of a non-affected control subject
Muhammad Zeeshan Afzal1, Melanie Gartz1, Ekaterina A Klyachko2
1Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
Stem Cell Research
|April 12, 2017
Abstract:
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.