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Related Experiment Videos

Brain Renin-Angiotensin System: Does It Exist?

Bibi S van Thiel1, Alexandre Góes Martini1, Luuk Te Riet1

  • 1From the Division of Vascular Medicine and Pharmacology, Department of Internal Medicine (B.S.v.T., A.G.M., L.t.R., D.S., E.U., I.M.G., F.P.J.L., A.H.J.D.), Department of Vascular Surgery (B.S.v.T., L.t.R., I.v.d.P., J.E.), Department of Molecular Genetics, Cancer Genomics Center Netherlands (B.S.v.T., I.v.d.P., J.E.), Division of Nephrology and Transplantation, Department of Internal Medicine (D.S., E.U.), Department of Radiation Oncology (J.E.), Erasmus MC, Rotterdam, The Netherlands; Department of Molecular Cardiovascular Endocrinology, Max Delbrück Center, Berlin, Germany (F.Q., N.A., M.B.); DZHK (German Center for Cardiovascular Research), Partner Site Berlin, Germany (N.A., M.B.); Berlin Institute of Health (BIH), Germany (M.B.); Charité-University Medicine, Berlin, Germany (M.B.); Institute for Biology, University of Lübeck, Germany (M.B.); Institute of Pathophysiology, Faculty of Medicine, Comenius University (L.P., R.R.); Institute of Normal and Pathophysiological Physiology, Slovak Academy of Sciences, Bratislava, Slovak Republic (L.P.); and Attoquant Diagnostics (O.D., M.P.) and Department of Internal Medicine III (O.D.), Medical University of Vienna, Austria.

Hypertension (Dallas, Tex. : 1979)
|April 12, 2017
PubMed

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Summary
This summary is machine-generated.

Brain renin activity is not locally synthesized but originates from trapped plasma renin. This suggests brain angiotensin II is absorbed from the blood, not produced within the brain.

Area of Science:

  • Neuroscience
  • Cardiovascular Physiology
  • Biochemistry

Background:

  • The brain renin-angiotensin system (RAS) is crucial for regulating physiological functions.
  • The blood-brain barrier suggests local synthesis of brain (pro)renin for RAS activity.
  • The angiotensin (Ang) I-generating activity (AGA) of intracellular brain renin remains uncharacterized.

Purpose of the Study:

  • To quantify brain (pro)renin and its Ang I-generating activity (AGA).
  • To determine the origin of brain renin and its contribution to brain Ang II.
  • To investigate the relationship between brain and plasma renin levels.

Main Methods:

  • Quantification of brain (pro)renin and AGA in various mouse models (wild-type, renin knockout, deoxycorticosterone acetate salt-treated, Ang II-infused).
Keywords:
DOCA saltSHRangiotensinbrainlisinoprilmouseolmesartanproreninrenin

Related Experiment Videos

  • Measurement of AGA before and after prorenin activation, using excess angiotensinogen and a renin inhibitor.
  • Assessment of AGA changes after buffer perfusion and comparison with plasma renin levels.
  • Analysis of Ang I and Ang II concentrations in spontaneously hypertensive rats.

    • Main Results:

    • Renin-dependent AGA was detected in multiple brain regions, with the highest activity in the brain stem.
    • Brain AGA was only marginally increased after prorenin activation, indicating low brain prorenin levels.
    • Buffer perfusion significantly reduced brain AGA (>60%), and brain renin levels correlated with plasma renin.
    • Brain renin levels were significantly lower than plasma renin levels, suggesting a non-local origin.
    • Ang I was undetectable in rat brains, and brain Ang II levels decreased after Ang II receptor blockade.

    Conclusions:

    • Brain renin is primarily trapped plasma renin, not locally synthesized.
    • Brain Ang II originates from circulating Ang II uptake rather than local synthesis.
    • These findings challenge the concept of an independent brain renin-angiotensin system.